Composite

Part:BBa_K2736106:Design

Designed by: Lorenzo Pattiradjawane   Group: iGEM18_Utrecht   (2018-10-12)


promoter Tar Q491A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 152


Design Notes

Durign the design of this part we decided to use a codon optimized mutation for E. coli UU1250, since that was the final chassis that we wanted to use for our system.


Source

This part was generated using site directed mutagenesis of BBa_2736105

References